Fall/Winter 2012

Swine Influenza Isolation Success

Following approval one year ago to begin manufacturing autogenous Swine Influenza (SIV) vaccines, Gallant has received over 47 cases for virus isolation. In many of these cases, Gallant has been successful in isolating SIV. Our procedure first includes identification of the virus of the original sample by PCR, and then subsequent testing on tissue culture to determine viability of the virus. Three sub- types have been found – H1N1, H3N2 and H1N2. It is important to stress the value of careful sampling and transport of specimens for virus isolation success. An ideal sample is from an animal that is shedding the virus and this may be from one that is not yet showing symptoms.

Successful production of vaccines has begun at Gallant.

On the Conference Trail

Jackie attended the Ontario Association of Swine Veterinarians conference in Guelph– October 12 to 13 and sponsored the lunch on Saturday.

Jackie travelled to Saskatoon October 24th to the Western Canadian Association of Swine Vets and gave a presentation on Emerging Trends and understanding new technology. Check out the website for a copy of the article included in the proceedings.

Haemophilus parasuis virotyping

Gallant is collaborating with Dr. Jan McInnis and Dr. Patrick Boerlin at the University of Guelph to identify virulence genes in Ontario Haemophilus parasuis isolates. Isolates from diseased and healthy pigs are screened using PCR to determine if they are positive for some of the known suspected virulence factors. Although identified in other countries, no other study had been conducted to determine if they were present in Canadian swine. The putative virulence genes are (vtaA1, HAPS_0254, nhaC, fhuA, wbgY, capD, hsdrR and fimB). There will also be an attempt to determine if there is a link between serotype and virulence genes. Early results indicate that Ontario isolates do contain these genes and they are present on isolates found in healthy and clinical cases.

Hog Jogging in Stratford 2012

Many of the Gallant Staff joined 435 other enthusiastic runners (walkers) on a hot day in June to raise money to purchase ovens for the Meals on Wheels programme. It is a fun event hosted by OPIC (Ontario Pork Industry Council). There was also 44 children running in the 500 metre "Weaner Run”. The event raised $46,000 for the cause. The 2013 Hog Jog run will be at the same venue in Stratford on June 19, 2013 in support of Victims Services through Janet O’Rourke.

Student Scholarships

The lab supports Veterinary students through scholarships at Ontario Veterinary College and the Atlantic Veterinary College in Prince Edward Island. This year Ms Meghan Levangie received the Gallant Custom Laboratories Anniversary Award at the October 4th ceremony in Charlottetown, PEI.

The award at OVC will be presented in March 2013.

Ontario Veterinary College Poultry and Swine Students Visit the Lab

A curious group of OVC students visited the lab in December to learn about autogenous biologics. The first group of 12 were from the Poultry Club and the second group of 4 were from the Swine Club. They enjoyed a pizza lunch, a seminar and then a tour of the facility.

They were enthusiastic and keen to learn about this option in animal health. The lab is always excited to show what we can do. We wish them all a bright future in the industry.

New PCR Test to Detect Swine Influenza

Guy Moser, Senior Scientist at Gallant Custom Laboratories, and consultant Dr. Steven Kleiboeker, have developed a real time PCR assay to detect the presence of the Swine Influenza virus (SIV) in swab and lung samples. This test is used as an initial screen to determine if the sample is positive for SIV by identifying the M gene – which is specific to SIV. This multiplex assay is unique because it also identifies if the virus is positive for H1, H3 and N2, in the same test. Future development work will attempt to add the N1 primer to the assay. This initial screen will be valuable to provide a quick answer to the presence and type of SIV circulating in the herd. The lab can utilize positive samples to isolate the virus if an autogenous vaccine is required. Our experience is indicating that there is a threshold of virus present, as indicated by the quantitative real time PCR assay, that will enable isolation in tissue culture ie the higher the virus concentration (lower the ct value) the greater the probability of isolating virus.